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Sorghum is cereal crop with a wide range of ago-ecological adaptation in Ethiopia. However, a number of biotic and abiotic factors are limiting grain yield increase. Diseases (leaf and grain) are considered as one of the major biotic factors hindering sorghum productivity in the highland and intermediate altitude sorghum growing areas of Ethiopia. In addition, the yield performance of crop varieties is highly influenced by genotype x environment (GxE) interaction which is the major focus of researchers while generating improved varieties. In Ethiopia, high yielding and stable varieties that withstand biotic stress in the highland areas are limited. In line with this, the yield performance of 21 sorghum genotypes and one standard check were evaluated across 14 environments with the objectives of estimating magnitude GxE interaction for grain yield and to identify high yielder and stable genotypes across environments. The experiment was laid out using Randomized Complete Block Design with three replications in all environments. The combined analysis of variance across environments revealed highly significant differences among environments, genotypes and GXE interactions of grain yield suggesting further analysis of the GXE interaction. The results of the combined AMMI analysis of variance indicated that the total variation in grain yield was attributed to environments effects 71.21%, genotypes effects 4.52% and GxE interactions effects 24.27% indicating the major sources of variation. Genotypes 2006AN7010 and 2006AN7011 were high yielder and they were stable across environments and they have been released for commercial production and can be used as parental lines for genetic improvement in the sorghum improvement program. Furthermore, GGE biplot also showed that the ideal genotype and ideal environment are Kulumsa 2011 and 96 AN 4020, respectively. In general, this research study revealed the importance of evaluating sorghum genotypes for their yield and stability across diverse highland areas of Ethiopia before releasing for commercial production.