15 / 21
Page 48
Food Science 2019
November 11-12, 2019
Food Science and Technology
November 11-12, 2019 | London, UK
3
rd
International Conference on
Volume 3
Applied Food Science Journal
Appl Food Sci J. | Volume 3
Novel detection protocol for radical scavenging and antioxidant activity of lipophilic antioxidants
Haue Md Azizul
Free University of Bozen-Bolzano, Italy
Statement of the problem:
Lipophilic antioxidants are an important class of chemical species of natural antioxidants
that can increase the oxidative stability of food matrices. Common methods to determine the antioxidant activity or
oxidative stress of lipophilic antioxidants require time consumingprotocols, although those arenot vigorously reliable.
Thisworkproposes a fast, simple anddirectmethodbasedon cyclic voltammetry tomonitor oxidation in lipid samples.
Methodology & Theoretical Orientation:
The oxidative stress during the reaction of AIBN (2, 2´-azobis
(20methylpropionitrile)) with lipid soluble antioxidants, such as α-tocopherol, catechin, retinyl acetate, caffeic acid
and 3-hydxoxytyrosol was evaluated. 1-propanol was used as a unique solvent, which allowed direct dissolution of
a wide range of lipid soluble redox species. Electron transfer (ET) capacity was evaluated by the peak current (ip)
and peak potential (E). The kinetic rate of the reactions between laboratory antioxidants andAIBN were measured at
60 °C. Finally, same procedure was also applied to measure the antioxidant activity and oxidative stress of different
commercialedibleoils:extravirginoliveoil(EVOO),virginoliveoil(VOO)and,olivebyproduct;sansaoliveoil(SOO).
Findings:
The methods demonstrated that antioxidant activity was positively correlated with increased concentrations
among the laboratory antioxidants and EVOO, VOO and SOO samples. On the other hand, oxidative stresses were
negatively correlated with the duration of reaction periods.
Conclusion & Significance:
This method can be the alternative of traditional methods to test lipid soluble antioxidants
in lipid matrices rapidly and straigh.