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Clin Cardiol J Volume 1 | Issue 1
December 04-05, 2017 Dallas, USA
International Conference on
Heart Congress, Vascular Biology and Surgeon’s Meeting
Endothelial intracellular molecule LSP1 regulates the chemotactic directionality of
extravascular migrating neutrophils
Liu Lixin
University of Saskatchewan, Canada
The recruitment of leukocytes from the flowing bloodstream into inflamed tissue is of key importance in
inflammation. This recruitment is characterized as the initial tethering and rolling of leukocytes along the
endothelium, followed by leukocyte activation and firm adhesion to the endothelium, leukocyte transmigration
across the endothelium(diapedesis), and chemotacticmigration of emigrated leukocytes toward the site of infection
or injury (chemotaxis) in tissue. The role of intracellular signaling molecules in leukocytes and endothelial cells
involved in leukocyte recruitment is investigated in the microvasculature in vivo. LSP1 (leukocyte-specific
protein 1), an F-actin-binding, intracellular phosphoprotein, is expressed in leukocytes and endothelial cells with
different intracellular localization pattern. In endothelial cells, LSP1 is mainly expressed in the nucleus with
small proportion as cytosolic and cytoskeletal protein. The role of endothelial LSP1 in neutrophil recruitment
is investigated and endothelial LSP1-regulated mechanism for extravascular neutrophil chemotaxis is revealed
in our study. Using intravital microscopy in LSP1-deficient and LSP1-chimeric mice, we show that endothelial
LSP1 plays a permissive role in controlling neutrophil transendothelial migration during neutrophil recruitment
and regulates extravascular migration directionality but not the migration velocity of emigrated neutrophils. We
found that the expression of α6β1 integrins on the emigrated neutrophils was blunted when LSP1 was deficient
in the endothelium of LSP1-deficient or chimeric mice, and that neutrophil α6β1 integrin expression dictated the
directionality of emigrated neutrophils in vivo and in vitro. LSP1-deficiency or LSP1-targeted siRNA silencing
in endothelial cells reduced endothelial adhesion molecule PECAM-1 expression through GATA-2-dependent
mechanism. LSP1 overexpression in endothelial cells has unregulated endothelial PECAM-1 expression. It
was the reduced endothelial PECAM-1 expression in LSP1-deficient endothelium that down-regulated α6 β1
integrin expression on the transmigrating neutrophils and that, through PECAM-1-sensitive, down-regulated
α6β1 integrin expression, mitigated the migration directionality of transmigrated neutrophils in tissue. Thus,
endothelial LSP1 regulates vascular PECAM-1-sensitive and neutrophil integrin α6β1 dependent directionality
of extravascular neutrophil migration in inflamed tissue during neutrophil recruitment.
Biography
Liu Lixin had research training with Dr. Dirk Roos (CLB, The Netherlands) and completed his PhD study with Dr. Per Venge at Uppsala University (Sweden).
In Europe, he has studied the regulatory mechanisms of granulocyte transmigration across epithelial cell monolayer. Thereafter, as a Post-doctoral Fellow, he has
joined the lab of Dr. Paul Kubes in the University of Calgary, in Alberta, Canada, where he became experienced in the techniques of intravital microscopy and
started to explore the role of intracellular protein molecules in neutrophil transendothelial migration and chemotaxis. In late 2006, he has joined the faculty in the
University of Saskatchewan (Saskatchewan, Canada) and currently he is Associate Professor in Pharmacology. Using intravital microscopy and other imaging
techniques and biochemical and cell biological approaches, his lab is now investigating the role of intracellular signaling molecules in neutrophil-endothelial
cell interactions, with current research interests in the signaling mechanisms of LSP1 (leukocyte-specific protein 1) and PI3K (phosphoinositide 3-kinases) in
neutrophil recruitment during inflammation.