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Page 39
3
rd
International Congress on
Annual Summit on
October 02-03, 2017 Atlanta,USA
&
Microbiology and Pharmaceutical Microbiology
Sexual & Reproductive Health
Journal of sexual and Reproductive medicine | Volume.1, Issue.2
Biochemical and molecular activities of
Candida albicans
treated with medicinal plants
Awatif Al-Judaibi
and
Sara Al-Alee
King Abdulaziz University, KSA
T
his study aimed to compare the antimicrobial activity of
Phoenix dactylifera and Ziziphus spina-christi
ethanol extracts in terms of their biochemical and molecular effects on
Candida albicans
. These effects were
evaluated regarding intracellular sterols, permeability of the cell membranes, and morphological characteristics
determined by scanning electron microscopy (SEM). Energy-dispersive X-ray spectroscopy (EDAX) analyses
were also conducted in addition to assessment of the changes in
TEF1: QRTTEF1, CaERG1: ERG1, CdERG12:
CdERG1,
and
ERG25: ERG25
genes. The results showed that sterols increased by 1.096% and 0.588% with
treatment by
P. dactylifera and Z. spina-christi
, respectively, compared to the untreated cells. The ethanol extracts
were effective on
C. albicans
permeability by reducing the cell membranes permeability. The SEM and EDAX
analyses showed cell cavities and shrinkage of the cell wall. In addition, the quantity of cells was decreased to a
few abnormal cells compared to the untreated cells. Yttrium was detected in the cells treated with
Z. spina-christi
,
and high levels of osmium were detected in the cells treated with P. dactylifera. The gene sequence showed gaps
and mismatches on
ERG1F, ERG1R, ERG12F, ERG12R
, and ERG25F genes after treatment with
P. dactylifera
and
Z. spina-christi
compared to untreated cells. The results were highly significant (p ≤ 0.01), and we concluded
that ethanol extracts of P. dactylifera and Z. spina-christi have an antimicrobial effect on several targets in yeast
cells.
tarawa62@hotmail.com