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Volume 3 Current Research: Integrative Medicine Chronic Diseases 2018
July 16-17, 2018
Chronic Diseases
July 16-17, 2018 Berlin, Germany
2nd International Conference on
Designing indirect ELISA test for detection of antibodies against serotype A2013 of Foot and Mouth
Disease (FMD) virus in cattle
Fatemeh Malekdar
Razi Vaccine and Serum Research Institute, Iran
Foot and Mouth Disease (FMD) virus is a contagious animal disease that causes irreparable damage to the economy of a country,
including Iran where the disease is native to. Among the ways to combat FMD is vaccination and slaughter. Because of the specific
situation of Iran, it is rather not possible to kill infected animals. Therefore, vaccination seems to be the most important way to fight
the disease. The methods used to evaluate the safety and determine the titer of antibody in a serum are mainly Serum Neutralization
Test (SNT) and Enzyme Linked Immune-Sorbent Assay (ELISA). In this research, designing an indirect ELISA test based on coating
of 140S complete viral particles makes it possible to determine antibody and following the fact that determining serotype and viral
type does not require time-consuming and complex molecular tasks, including gene expression. In addition, in the event of a new
epidemic, a new epidemic condition can be detected by using serum antibody method. However, coating complete viral particle leads
requires virus purification as well as anti-immunoglobulin conjugate antibody testing of the same animal. In this study, SNT test was
used as a Gold Test to determine the serum antibody level and its results was compared with indirect ELISA method to determine the
sensitivity and specificity of the indirect ELISA test for measuring the anti-virus antibody rate of type (A2013) FMD through ROC
analysis, with 100% sensitivity and the specificity of 90% sensitivity, using routine formulas with 100 sensitivity and specificity of 82%.
In this study, considering a cut off OD=0.3, there was a significant difference between the vaccinated animals and the unvaccinated
animals in terms of antibody level against the A2013 type. This indicates the correctness of the test and the accurate and proportional
antibody detection against the under study viral types of FMD.
Biography
Fatemeh Malekdar is a Research Assistant at the Razi Vaccine and Serum Research Institute. Her main research interests are in FMD virus. She is currently
working on MSc thesis on the Designing Indirect ELISA Test for Detection of Antibodies against Serotype A2013 and O2010 of Foot and Mouth Disease (FMD) virus
in cattle. The research is supervised by Professor Homayoon Mahravani in FMD department.
Fatemeh Malekdar, Curr Res Integr Med 2018, Volume 3
DOI: 10.4172/2529-797X-C1-002