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Volume 1, Issue 1 Journal of General Dentistry Euro Dental Market 2017
December 07-08, 2017
DENTAL MARKETING
December 07-08, 2017 | Madrid, Spain
27th Global Summit and Expo on
Nunthawan Nowwarote, Journal of General Dentistry 2017, 1:1
Basic fibroblast growth factor regulates pyrophosphate/phosphate regulating gene expression in stem cells
isolate from human exfoliated deciduous teeth
Nunthawan Nowwarote
Chulalongkorn University, Thailand
Basic fibroblast growth factor (bFGF) play roles in stem cells isolate from human exfoliated deciduous teeth (SHEDs) maintenance
and regulated cells differentiation. Moreover, bFGF signaling have an effect on mineralization by regulate pyrophosphate/
phosphate balance in cells. From our previous study showed that under osteogenic differentiation, bFGF decreased ALP activity and
mRNA expression also reduced nodule formation. The aim of present study was investigated the regulation of bFGF on osteogenic
differentiation in SHED via pyrophosphate/phosphate regulating gene expression. SHEDs expressed mesenchymal stem cells
surface markers (CD44, CD90, CD105) and differentiated into osteogenic and adipogenic lineage upon differentiation medium
cultured. Exogenous bFGF reduced ALP mRNA expression and protein levels also inhibit bone nodule formation in osteogenic
induction. SHEDs were maintained in osteogenic induction after 14 days, did not showed effect on PPi/Pi regulating gene (ANKH,
ENPP-1 and PIT-1), in contrast, bFGF mRNA expression was significantly decreased at 14 days and ALP mRNA expression was
significantly increased at 3 and 7 days. Treating SHEDs with exogenous bFGF reduced ALP mRNA expression and significantly
increased ANKH mRNA expression and this effect was rescued ALP and ANKH mRNA expression by FGFR inhibitor. Further, the
intracellular mechanism implying the involvement of intermediate molecules pretreated with FGFR inhibitor and cycloheximide
were up-regulated ALP mRNA expression and down-regulated ANKH mRNA expression at 24 hours. Notably, treating SHEDs with
phosphate were enhanced calcium accumulation upon osteogenic induction 14 days whereas treating SHEDs with pyrophosphate
were inhibited. In conclusion, bFGF enhanced Pi/PPi regulating gene expression in SHEDs.
Biography
Nunthawan Nowwarote has completed her PhD at faculty of Dentistry, Chulalongkorn University, Thailand. She has worked at mineralize tissue research unit at
faculty of dentistry, Chulalongkorn University for six years. She got poster award: 6th Hiroshima Conference on Education and Science in Dentistry 2015, 21-30
October 2015 at Hiroshima Japan by oral presentation and poster presentation in title basic fibroblast growth factor induced interleukin six expression by stem cell
isolated from human exfoliated deciduous teeth.