stem-cells-2019-posters-abstracts

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Volume 02

Stem Cells 2019 & Pediatrics Congress 2019

November 06-07, 2019

Journal of Clinical Genetics and Genomics

November 06-07, 2019 | Tokyo, Japan

STEM CELLS AND REGENERATIVE MEDICINE

PEDIATRICS AND CHILD CARE

International Conference on

World Congress on

J Clin Gen Genomics, Volume 02

Liver tissues regenerated from human tooth treats liver failure of rat cirrhosis model

and swine NASH model

K Yaegaki

and

H Ishikawa

Nippon Dental University, Japan

adaveric or live-donor liver transplant is only the treatment for severe liver condition. However, the number of transplantations

is very limited because of fewer available organs than number of the patients on the waiting list. The liver regeneration might be

one of the alternative. Several clinical studies employed mesenchymal stem cells from blood, adipose tissue or others to transplant

without differentiating the cells. However, Transplantation of these cells can only slow decline of hepatic function, but they cannot

treat the conditions of the liver. The objective of adult stem cell transplantations might be to launch “bridge to transplant” strategy

rather than treating liver condition. We have shown that human dental pulp stem cell demonstrates huge potential to treat lethal liver

conditions. We have previously reported we treated the biliary liver cirrhosis and acute liver injury in nude rats with transplanting the

regenerated liver tissues originated from human dental pulp. One of the most prevailing liver condition is non-alcoholic steatohepatitis

(NASH). Hence the objectives of the research is to evaluate the clinical possibility of our transplantation protocols using swine model

of progressive liver failure developed from NASH. After four weeks of transplantation of hepatocytes described from human tooth

into the spleen of 6 swine with the failure under immune suppression, we found the secondary liver in the spleen was produced, as well

the regenerated liver was produced using the original liver as scaffold. Biliary ducts are reproduced with human tissues only 4 weeks

after the transplantation. Serum albumin level recovered from 1.5g/dL to over 3.0g/dL. HPT, choline esterase, collagen type IV, ALT

and others have been dramatically improved. But any of the positive control has shown no change.

Following above we also treated rat cirrhosis model.