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Volume 3
August 5-6, 2019 | Singapore
CANCER RESEARCH AND PHARMACOLOGY
STRUCTURAL BIOCHEMISTRY, STEM CELLS AND MOLECULAR BIOLOGY
24
th
International Conference on
International Congress on
&
Cancer Research 2019 & Structural Biochemistry 2019
August 5-6, 2019
Journal of Cancer and Metastasis Research
Immobilization of antitumor enzyme L-lysine alpha-oxidase from Trichoderma cf.
aureoviride Rifai on the nanocomposite polyGraphene as matrix for cancer screening,
diagnosis and treatment
Dmitry Malenkov
1
, A S Botin
2
, A Yu Arinbasarova
3
, A G Medentsev
3
, T S Popova
4
, I P Smirnova
5
1
Scientific Center of Integrative Medicine, Russia
2
Sklifosovsky Institute of Emergency Medicine, Russia
3
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russia
4
Sklifosovsky Institute of Emergency Medicine, Russia
5
RUDN University, Russia
I
t was discovered that expanded graphite – Nanocomposite of PolyGraphene (PG) obtained after hydro-termic treatment of
modified graphite became to be able to interact as sorbent PG with enzyme L-lysine alpha-oxidase from Trichoderma cf.
aureoviride Rifai.
It was studied the sorption properties of carbon material as an example of PolyGraphene (PG) concerning enzyme L-lysine alpha-
oxidase from Trichoderma cf. aureoviride Rifai.
PG - version of ultrafine carbon sorbent, which was developed on the basis of the modified oxygen-containing expanded graphite
(OCEG).
L-Lysine α-oxidase (LysOx) is one of the enzymes which are prospective in biotech-nology and medicine due to its antitumor
and kinetic properties. An
in vivo
therapeutic effect was demonstrated on animals with tumor grafts: breast carcinoma SKBR3,
Bro melanoma, intestinal cancer HCT116 and LS174T, ovary adenocarcinoma SCOV3, liver carcinoma.
This work aims to immobilize the extracellular L-lysine α-oxidase (LysOx) from Trichoderma cf. aureoviride Rifai VKM
F-4268D on PolyGraphene and characterize some properties of adsorbed enzyme. Two types of PolyGraphene were used.
Maximum adsorption equal to 5 or 11 μg protein/ mg of carrier was achieved, a high specific activity comparable to that of a
free enzyme to take place.
LysOx adsorbed on PolyGraphene was shown to be a very stable system, namely high stability was revealed in the presence of
chaotropic agent (urea) or proteolytic enzymes (pronase, chymotrypsin, trypsin).
Thus, the possibility of immobilization of LysOx on graphene with the full conservation of specific activity was shown.
In addition, LysOx is one of the enzymes that is promising in the enzyme therapy of tumors, based on the high sensitivity of
tumor cells to the deficiency of growth factors, including amino acids.
Conclusions. Researchers have the theoretical and practical importance, the received results can be applied as matrix both in
cancer screening, diagnosis and treatment and in cleaning of already known enzymes.
Biography
Dmitry Malenkov is now studying Public health management and Economics in Higher School of Economics. Surgeon, cardiothoracic
surgeon, oncologist, scientist, public health administrator, co-founder of Scientific center for integrative medicine. Dmitry Malenkov is
the author of more than 40 papers, in the field of clinical and experimental medicine.
drmalenkov@gmail.com