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Journal of Neurology and Clinical Neuroscience | Volume: 03
8
th
International Conference on
NEUROLOGICAL DISORDERS,
CENTRAL NERVOUS SYSTEM AND STROKE
&
International Conference on
NEUROLOGY AND NEUROSURGERY
December 04-05, Dubai, UAE
Joint event on
J Neurol Clin Neurosci, Volume: 03
Mat r Sci Nanot chnol
A novel device to simulate traumatic Brain injury
Krystle Karoscik
Neuro StartUp Challenge, USA
T
raumatic brain injury (TBI) is a major health concern
in children and adults as it has been proposed as a
risk factor for the development of subsequent neurologic
diseases that often lead to irreversible brain damage
or death. A meta-analysis of TBI identified several
key associations, notably etiological pathology and
complications from the nature of the injury, and various
clinical presentations. The exact mechanism of cellular
injury is not well understood. This novel device allows for
varied shockwave propagation to simulate cellular injury
and independently study the role of shockwave pressure
change and shear force damage. The purpose of this device
is to determine the overall consequences of traumatic
exposure to brain tissue, and to provide a system in which
tissue could be directly observed during and immediately
after exposure to shockwave propagation. The pneumatic
air-gun based device delivers a blast via a quick release
valve directly to the 96-well culture plate positioned on
top of a microscope. Modulating the volume of fluid in
the well allows for independent control over shear forces
generated by the blast shockwaves. The device is used in
a laboratory controlled system with high temporal and
spatial resolution. Novelties include real-time cellular
imaging and analysis of explosive shockwaves, screening
for pharmacological compounds that may ameliorate
the effects of a brain trauma, testing materials capable
of protecting cells from trauma, and identifying the best
treatment and diagnostic path based on injury from
head trauma. Future applications will be used to study
pharmacological effects of calcium signaling in response
to trauma, search for additional signaling pathways in
response to varying intensities, and expand the system to
allow for study of entire organs.
e:
krystle.kar@gmail.com