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Volume 2

Journal of Microbiology and Biotechnology Reports

Microbial Biotechnology 2018

September 17-18, 2018

Microbial Biotechnology & Vaccine Design

September 17-18, 2018 Lisbon, Portugal



World Congress on

Transposon insertion sequencing identified a novel horizontal transfer nucleoid associated protein

regulating virulence

Ruiqing Ma and Qiyao Wang

East China University of Science and Technology, China

Horizontal acquired genes play important roles in bacterial chromosome evolution and they are typically grouped together in

blocks termed genomic islands. Transposon Insertion Sequencing (TIS) is a powerful high-throughput genetic technology that

facilitates exploration of conditionally essential genes. Using this technique, a horizontal acquired gene named enrR was identified in

Edwardsiella piscicida

, which is one of the chief infectious threats for farm-raised fish. The mutant of enrR can decrease the expression

of whole T3SS and T6SS gene islands, thereby attenuate its virulence in host. With comprehensive analysis of its function by ChIP-

seq and RNA-seq, we discovered an interesting phenomenon that the working areas of protein EnrR in genome are closed to GIs

(genomic islands) and EnrR can repress their expression by binding to their nearby regions. We finally identified EnrR as a nucleoid

associated protein with non-specific DNA binding ability and DNA aggregation ability

in vitro

and its binding ability with DNA is

dependent on the length of DNA fragments. As a horizontal transfer regulator, EnrR can also enhance virulence in Salmonella. Our

study discovered a universal significant horizontal-transferred virulence activator, which deepens our understanding for bacterial

virulence evolution.


Ruiqing Ma is a PhD student of East China University of Science and Technology focusing on marine pathogenic microorganism. He devotes to identify new

virulence regulator using sequencing technology including TIS, ChIP-seq and RNA-seq, which is used for developing new attenuated live vaccine. His project is

focused on Edwardsiella piscicida that is a bane for aquaculture industry.

Ruiqing Ma et al., J Microbio and Biotech Rept 2018, Volume 2