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Page 38


Volume 2

Journal of Microbiology and Biotechnology Reports

Microbial Biotechnology 2018

September 17-18, 2018

Microbial Biotechnology & Vaccine Design

September 17-18, 2018 Lisbon, Portugal



World Congress on

Production of poly-3-hydroxybutyrate and oxygen transfer characterization by

Azotobacter vinelandii


a 30L bioreactor

Andres Perez and Alvaro Diaz-Barrera

Pontificia Universidad Católica de Valparaíso, Chile

Polyhydroxybutyrate (PHB) is a biopolymer produced by

Azotobacter vinelandii

. The PHB is a biodegradable thermoplastic material,

used in packaging production, drug encapsulation and medical implants. The objective of this work was to develop a PHB production

process in 30L bioreactor. Cultures of

A. vinelandii

OP were performed in batches in an Infors HT stirred tank reactor model

Techfors-S with 20L of culture medium composed of sucrose as a carbon source and yeast extract as nitrogen source. The culture was

developed at 100 rpm, 1 vvm, 30 °C and pH 7.1 controlled with 2N NaOH. The Dissolved Oxygen Tension (DOT) was characterized

with a polarographic sensor and the Oxygen Transfer Rate (OTR) was estimated by analyzing O2 and CO2 in the gas phase. The

results show that the biomass reached a maximum value of 7.5g L-1 after 70 hours of cultivation. Sucrose was completely consumed,

indicating that it was the nutrient limiting growth. The DOT remained at values close to zero during the cell growth phase and the

OTR reached a constant maximum value for 30 hours, reaching 10 mmol L-1h-1. This OTR behavior is typical of oxygen-limited

cultures. The concentration of PHB was increased during cell growth, until reaching a maximum concentration of 6.0 g L-1. Likewise,

the percentage of intracellular accumulation of PHB varied between 65% and 82% between 35 and 50 hours of culture. Results of

PHB are like that reported by Millán et al., in 2017 with percentages of accumulation of constant PHB over 70%. YPHB/S of 0.24

gg-1 with qPHB of 0.01 gg-1h-1. Our results have demonstrated it was possible to develop a PHB production process on a 30L scale

getting PHB concentrations of 6 g L-1. Furthermore, by characterizing the oxygen transfer it is possible to explain the high percentage

of PHB accumulated by

A. vinelandii



Andres Perez is a student of Master of Science of Engineering in Biochemical Engineering at Pontificia Universidad Católica de Valparaíso, Chile. His profession

is a Biotechnologist Engineer of Concepcion University of Chile.

Andres Perez et al., J Microbio and Biotech Rept 2018, Volume 2