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Page 45


Volume 2

Journal of Microbiology and Biotechnology Reports

Microbial Biotechnology 2018

September 17-18, 2018

Microbial Biotechnology & Vaccine Design

September 17-18, 2018 Lisbon, Portugal



World Congress on

Maria Camila Escobar et al., J Microbio and Biotech Rept 2018, Volume 2

Reduction of selenite to selenium using bacteria isolated from polluted areas

Javed Saima


, Faisal Muhammad


and Naeem Muhammad Salman



University of the Punjab, Pakistan


National Textile University, Pakistan

In this work toxic selenite which is also highly soluble is transformed to selenium a less toxic element. Selenium is transformed through

biotic transformation using different strains of bacterial like

Pseudomonas, Exiguobacterium sp


Bacillus subtilis




Selenium can exist in different forms like reduced form (Selenide, Se2-), water dissolved form (Selenite, So3-2/Selenate SeO4-2) and

in the form of element (Seo). Different physical parameters were changed for optimizing conditions like different concentrations of

Selenium (Se) varying from 200 to 400 and finally to 600 µg ml-1, temperature, pH, aeration along with incubation time for high

reduction of selenite. It was found that selenite reduction rate was increased by increasing pH. It was found that at pH 3 around 15-

33% Selenite was reduced and this trend kept on increasing to 28-90% at pH 9. For evaluating optimum temperature for Selenite

reduction three levels of temperature were selected (32 °C, 37 °C and 42 °C) were selected. The selenite reduction was found at

different temperatures and the results showed that for optimum reduction of selenite all strains possess varying preferences. The

reduction in Selenite was also checked at different concentrations of selenite and it was found that maximum reduction of selenite

was observed at lower concentration. This study concluded that in aerobic and anaerobic environment Se can be remediated by using

selenite reducing bacteria.