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Volume 2

Journal of Microbiology and Biotechnology Reports

Microbial Biotechnology 2018

September 17-18, 2018

Microbial Biotechnology & Vaccine Design

September 17-18, 2018 Lisbon, Portugal



World Congress on

Prevalence and control of

Cronobacter sakazakii

containing putative virulence genes

Shruti Sharma and Alka Prakash

Dayalbagh Educational Institute, India

Cronobacter sakazakii

is an emerging food-borne pathogen that is associated with a number of infections in infants, neonates

and immune-compromised individuals. It is associated with a number of life-threatening diseases like necrotizing enterocolitis,

sepsis, meningitis, bacteremia, CSF infection, brain abscess and cyst formation. In the present study prevalence of C. sakazakii was

investigated in dairy products of Agra city, India. Total 243 dairy product samples were analyzed in different seasons and a total of 480

isolates were obtained. These isolates were biochemically characterized and were further confirmed using 16S rRNA gene sequencing.

The isolates were subjected to

in vitro


in vivo

pathogenicity testing. The presence of virulence associated genes was checked in

these isolates and the effect of various stresses like acid, alkaline, heat, cold and desiccation was also studied on these isolates. qPCR

based studies were conducted to check the expression of putative virulence gene under stressed and unstressed cells. A number of

antibiotics and probiotics were tested to control these

C. sakazakii

isolates. Amongst antibiotics Ofloxacin, Piperacillin, Cefotaxime

and Chloramphenicol were the most effective antibiotics and amongst probiotics

L. fermentum


Pediococcus acidilactici

were the

most effective in controlling the

C. sakazakii


Shruti Sharma et al., J Microbio and Biotech Rept 2018, Volume 2