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Page 48
Volume 2
Journal of Microbiology and Biotechnology Reports
Microbial Biotechnology 2018
September 17-18, 2018
Microbial Biotechnology & Vaccine Design
September 17-18, 2018 Lisbon, Portugal
5
th
World Congress on
Antibacterial activity of methanol extract of Mazouj and Ghalghaf galls extracts of Oak against
Pseudomonas aeruginosa
Shideh Fatehi
1
, Maryam Mohammadi Sichani
1
and Majid Tavakoli
2
1
Islamic Azad University, Iran
2
Agricultural & Natural Resources Research Center of Lorestan, Iran
Background & Aim:
Most of the infections caused by
Pseudomonas aeruginosa
resistant strains are originated from hospitals and
its prevalence is increasing worldwide. Therefore, many efforts have been made to find new effective plant compounds as a substitute
for antibiotics. The aim of this study is to evaluate the active constituents, antibacterial and anti-biofilm activity of aqueous and
methanol extracts of Mazuj and Ghalghaf galls against
Pseudomonas aeruginosa
.
Method:
This study was performed by standard strains of bacteria. The methanol extract of Mazuj and Ghalghaf galls were prepared
by Soxhlet apparatus. Antibacterial activity of extracts was evaluated by the diffusion method. Moreover, Minimum Inhibitory
Concentration (MIC) was assessed by micro dilution method. In order to evaluate anti-quorum sensing activity of methanol and
aqueous extracts of Mazuj and Ghalghaf galls, effects of the extract on anti-biofilm activity, production of elastase, proteases and
pyocyanin were examined. Active compounds Mazuj and Ghalghaf galls were identified by gas chromatography-mass.
Results:
The methanol and aqueous extracts of Mazuj and Ghalghaf galls exhibited strong inhibitory effects against
Pseudomonas
aeruginosa
. The MIC values of extracts were similar and ranged from 6.25 mg/ml to 25 mg/ml. The extracts of Mazuj and Ghalghaf
galls strongly inhibited the formation of
Pseudomonas aeruginosa
biofilms, production of elastase, proteases and pyocyanin at
concentrations higher than 6.25 mg/ml. The highest compound isolated from the extract of Mazuj galls 9-Octadecenoic acid and the
extract of Ghalghaf galls 9-12 octadecenoic acid and oleic acid.
Conclusion:
According to the results of this study, these extracts demonstrate favorable antibacterial activity against tested bacteria.
The methanol extract proved to have more antibacterial activity than the aqueous extract. However, a profound understanding of the
field identification of antimicrobial agents in diseases treatment as the alternative to antibiotics requires more experimental research.
shideh_fatehi@yahoo.comShideh Fatehi
et al., J Microbio and Biotech Rept 2018, Volume 2