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Page 48

Volume 2

Journal of Microbiology and Biotechnology Reports

Microbial Biotechnology 2018

September 17-18, 2018

Microbial Biotechnology & Vaccine Design

September 17-18, 2018 Lisbon, Portugal



World Congress on

Antibacterial activity of methanol extract of Mazouj and Ghalghaf galls extracts of Oak against

Pseudomonas aeruginosa

Shideh Fatehi


, Maryam Mohammadi Sichani


and Majid Tavakoli



Islamic Azad University, Iran


Agricultural & Natural Resources Research Center of Lorestan, Iran

Background & Aim:

Most of the infections caused by

Pseudomonas aeruginosa

resistant strains are originated from hospitals and

its prevalence is increasing worldwide. Therefore, many efforts have been made to find new effective plant compounds as a substitute

for antibiotics. The aim of this study is to evaluate the active constituents, antibacterial and anti-biofilm activity of aqueous and

methanol extracts of Mazuj and Ghalghaf galls against

Pseudomonas aeruginosa



This study was performed by standard strains of bacteria. The methanol extract of Mazuj and Ghalghaf galls were prepared

by Soxhlet apparatus. Antibacterial activity of extracts was evaluated by the diffusion method. Moreover, Minimum Inhibitory

Concentration (MIC) was assessed by micro dilution method. In order to evaluate anti-quorum sensing activity of methanol and

aqueous extracts of Mazuj and Ghalghaf galls, effects of the extract on anti-biofilm activity, production of elastase, proteases and

pyocyanin were examined. Active compounds Mazuj and Ghalghaf galls were identified by gas chromatography-mass.


The methanol and aqueous extracts of Mazuj and Ghalghaf galls exhibited strong inhibitory effects against



. The MIC values of extracts were similar and ranged from 6.25 mg/ml to 25 mg/ml. The extracts of Mazuj and Ghalghaf

galls strongly inhibited the formation of

Pseudomonas aeruginosa

biofilms, production of elastase, proteases and pyocyanin at

concentrations higher than 6.25 mg/ml. The highest compound isolated from the extract of Mazuj galls 9-Octadecenoic acid and the

extract of Ghalghaf galls 9-12 octadecenoic acid and oleic acid.


According to the results of this study, these extracts demonstrate favorable antibacterial activity against tested bacteria.

The methanol extract proved to have more antibacterial activity than the aqueous extract. However, a profound understanding of the

field identification of antimicrobial agents in diseases treatment as the alternative to antibiotics requires more experimental research.

Shideh Fatehi

et al., J Microbio and Biotech Rept 2018, Volume 2