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Journal of Reproductive Biology and Endocrinology

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Nuñez-Calonge Rocío, PhD1,2, Rancan Lisa, PhD2, Cortés Susana, PhD3, Vara Elena, MD, PhD4, Andrés Carolina, PhD3, Caballero Pedro, MD1 and Fernández-Tresguerres Jesús, MD, PhD2
 
1 Fundacion Tambre, Madrid, Tambre 8, 28002 Madrid, Spain, Email: rocioncalonge@gmail.com
2 Department Physiology, Medical School, University Complutense of Madrid, Spain, Email: lisaranc@ucm.es
3 Clinical Tambre, Madrid, Tambre 8, 28002 Madrid, Spain, Email: susana.c@gmail.com
4 Departments of Biochemistry and Molecular Biology Medical School, University Complutense of Madrid, Spain, Email: Vara.elena@ucm.es
 

Received: 19-Jan-2019 Accepted Date: Jan 22, 2019; Published: 31-Jan-2019

Citation: Nuñez-Calonge R, Rancan L, Cortes S, et al. Apoptotic markers and antioxidant enzymes have altered expression in cumulus and granulosa cells of young women with poor response to ovarian stimulation. J Reprod Biol Endocrinol. 2019;3(1):1-6.

This open-access article is distributed under the terms of the Creative Commons Attribution Non-Commercial License (CC BY-NC) (http://creativecommons.org/licenses/by-nc/4.0/), which permits reuse, distribution and reproduction of the article, provided that the original work is properly cited and the reuse is restricted to noncommercial purposes. For commercial reuse, contact reprints@pulsus.com

Abstract

Background: The objective of this study was to evaluate apoptosis and oxidative stress markers in Granulose (GC) and Cumulus Cells (CC) of women with low response to Controlled Ovarian Stimulation (COS) and controls which underwent an IVF treatment.

Methods: mRNA concentrations of BCL-2, BAX, caspase-3, caspase-9, Superoxide Dismutase (SOD) and catalase were evaluated in patients (n=30) and donors (n=45) using qRT-PCR analysis on CG and CC.

Results: The concentrations of mRNA coding for BCL-2, BAX, caspase-3, and caspase-9 were significantly higher in GC from women with low response compared to women with normal response (p<0.001). In contrast, concentrations of catalase mRNA were significantly increased in normoresponders women (p< 0.05). The levels of catalase mRNA were also significantly higher in CCs from normoresponders women compared to patients with low response (p<0.003), and the levels of BAX mRNA in CC were significantly increased in patients (p<008).

Conclusions: In addition, we found a correlation between the expression of genes regulating OS and apoptosis and response to ovarian stimulation, which could mean that an increase in ovarian oxidative stress can reduce the number of oocytes obtained after ovarian stimulation.

 
Google Scholar citation report
Citations : 18

Journal of Reproductive Biology and Endocrinology received 18 citations as per Google Scholar report

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