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Electromyography is a biomedical technique that examines muscle function by analyzing electrical signals generated during muscle contractions. Signal recording of the electromyogram is done using a device called an electromyograph. This device reveals the electrical potentials created by the muscle cells in both surface and interior method. This signal can be used in various fields such as medical and rehabilitation studies. To develop and validate bioelectric signal measurement systems, there will be an accurate validation method. If the system is being developed, its validation in several stages will increase the cost of this test, but the costs can be significantly reduced with the help of a software simulator. In this paper, we investigate and simulate a simple EMG Simulator in LabVIEW Software.