All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
Journal of Pharmacology and Medicinal Chemistry

Sign up for email alert when new content gets added: Sign up

Assessment of cell viability in an astrocyte cell line exposed to amyloid beta peptide and Fasudil

Author(s): Burçin Nilay Yener*, Necla Benlier and Hülya Çicek

Background: Amyloid beta (Aβ) is a protein of 40-42 aminoacids that are crucially involved in Alzheimer’s disease as the main component of amyloid plaques. Rho-kinase is believed to be involved in the regulation of stress fiber formation, smooth muscle contraction, cell migration and proliferation. Rho-kinase inhibition results in reduced inflammatory response. Fasudil is a ROCK inhibitor. In the present study, we aimed to evaluate cell viability in an astrocyte cell line treated with amyloid beta peptide and fasudil.

Methods and results: C8-D1A (a murine astrocyte cell line) was used to construct a neurotoxicity model induced by Aβ peptide. Astrocyte cells were divided into 2 groups including control and amyloid beta groups and incubated with 5 μM amyloid beta for 24 hours. A separate group was treated with 2.5 μM dose of Fasudil, a Rho-kinase inhibitor, and photographs of the resulting cell samples were taken with 40X magnification objective of the inverted microscope.

A marked increase in cell loss was observed in the group exposed to 5 μM Aβ after incubation in comparison to control group (p<0.001). Fasudil was found to prevent cell loss considerably in the group treated with Fasudil 2.5 μM and Aβ compared to the group treated with Aβ alone (p<0.001).

Conclusion: In the present study, we found that Aβ caused abundant cell death in the murine C8-D1A astrocyte cell line and Fasudil effectively prevented cell loss induced by amyloid beta.


Full-Text | PDF

Recommended Conferences

Global Summit on Pharmaceutics & Nanomedicine

London, UK
Top