Concentrated antibody standardization for use in automated immunohistochemistry

Immunohistochemistry (IHC) is a diagnostic and research tool for detecting proteins in cells or tissues. It has acquired an essential supporting function in the molecular diagnosis of specific neoplasias in modern pathology, with an exponential contribution in customized therapy. Through standardization, aut- -omation in immunohistochemistry assists to lower test variability. The transition to automation is a process, and if a laboratory already has a collection of stock concentrated primary antibodies, these antibodies should ideally be compatible with the chosen automated method, as antibodies are too valuable to be discarded as a result of the migration to automated immunohistochemistry.