Journal of Agriculture

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DKW emerges as a superior media factor in in vitro plant regeneration

Author(s): Shafkat Shamim Rahma

Plant regeneration heavily depends on the basal media factor. Recent studies showed the potential role of Driver and Kuniyaki Walnut (DKW) medium as an alternative to popular MS medium. In these studies, DKW distinctly depicted superiority over MS medium in terms of multiple shoot induction and callus mediated shooting of C. ternatea and B. australis. Earlier reports also claimed, the successful regeneration studies of Pimpinella pruatjan, Theobroma cacao, Manihot esculenta Crantz, Corylus avellana, Juglans regia, Juglans nigra with a high percentage of germination and plantlet conversion in DKW medium. The disparity between DKW and MS media is mostly in their macronutrients composition, but the solidifying agent Gelrite in DKW is also assumed to be a key factor to separate them in terms of success


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