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Single Cell (SC) examinations of key undeveloped, fetal and grown-up stages were performed to create a thorough single cell map book of all the corneal and adjoining conjunctival cell types from advancement to adulthood. Four human grown-up and seventeen early stage and fetal corneas from 10 to 21 Post Origination Week (PCW) examples were separated to single cells and exposed to scRNA-and additionally ATAC-Seq utilizing the 10x Genomics stage. These were implanted utilizing Uniform Manifold Approximation and Projection (UMAP) and bunched utilizing Seurat chart based grouping. Bunch recognizable proof was performed in light of marker quality articulation, bioinformatics information mining and Immunofluorescence (IF) investigation. RNA impedance, IF, state shaping effectiveness and clonal tests were performed on refined Limbal Epithelial Cells (LECs).