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MEscherichia coli, a gut flora, is increasingly responsible for urinary tract infection in the community and hospital settings. The antibiotic resistance of E. coli, particularly the production of extended-spectrum beta-lactamases (ESBLs) which confer resistance to extended spectrum cephalosporins and monobactam challenges public health issue and creates increasing difficulty in the diagnosis and therapeutic options. In this study, we have detected the presence of multidrug resistant E. coli harboring blaSHV associated with urinary tract infection. This is a prospective study performed in the patients who had attended two different primary health care settings (Kalinagar and Girishganj) of Assam, north eastern India from July 2019 – February 2020. A total number of 246 midstream urine samples were collected from patients suffering from UTI or suspected to have UTI. The significant bacteriuria was determined by culture loop method.