Basic fibroblast growth factor regulates pyrophosphate/phosphate regulating gene expression in stem cells isolate from human exfoliated deciduous teeth

27^th Global Summit and Expo on Dental Marketing

December 07-08, 2017 | Madrid, Spain

Nunthawan Nowwarote

Chulalongkorn University, Thailand

Posters & Accepted Abstracts: J Dent and Oral Res

Abstract :

Basic fibroblast growth factor (bFGF) play roles in stem cells isolate from human exfoliated deciduous teeth (SHEDs) maintenance and regulated cells differentiation. Moreover, bFGF signaling have an effect on mineralization by regulate pyrophosphate/ phosphate balance in cells. From our previous study showed that under osteogenic differentiation, bFGF decreased ALP activity and mRNA expression also reduced nodule formation. The aim of present study was investigated the regulation of bFGF on osteogenic differentiation in SHED via pyrophosphate/phosphate regulating gene expression. SHEDs expressed mesenchymal stem cells surface markers (CD44, CD90, CD105) and differentiated into osteogenic and adipogenic lineage upon differentiation medium cultured. Exogenous bFGF reduced ALP mRNA expression and protein levels also inhibit bone nodule formation in osteogenic induction. SHEDs were maintained in osteogenic induction after 14 days, did not showed effect on PPi/Pi regulating gene (ANKH, ENPP-1 and PIT-1), in contrast, bFGF mRNA expression was significantly decreased at 14 days and ALP mRNA expression was significantly increased at 3 and 7 days. Treating SHEDs with exogenous bFGF reduced ALP mRNA expression and significantly increased ANKH mRNA expression and this effect was rescued ALP and ANKH mRNA expression by FGFR inhibitor. Further, the intracellular mechanism implying the involvement of intermediate molecules pretreated with FGFR inhibitor and cycloheximide were up-regulated ALP mRNA expression and down-regulated ANKH mRNA expression at 24 hours. Notably, treating SHEDs with phosphate were enhanced calcium accumulation upon osteogenic induction 14 days whereas treating SHEDs with pyrophosphate were inhibited. In conclusion, bFGF enhanced Pi/PPi regulating gene expression in SHEDs.

Biography :

Nunthawan Nowwarote has completed her PhD at faculty of Dentistry, Chulalongkorn University, Thailand. She has worked at mineralize tissue research unit at faculty of dentistry, Chulalongkorn University for six years. She got poster award: 6th Hiroshima Conference on Education and Science in Dentistry 2015, 21-30 October 2015 at Hiroshima Japan by oral presentation and poster presentation in title basic fibroblast growth factor induced interleukin six expression by stem cell isolated from human exfoliated deciduous teeth.