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Journal of Cancer & Metastasis Research

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Immobilization of antitumor enzyme L-lysine alpha-oxidase from Trichoderma cf. aureoviride Rifai on the nanocomposite polyGraphene as matrix for cancer screening, diagnosis and treatment

Joint Event on 24th International Conference on CANCER RESEARCH AND PHARMACOLOGY & International Congress on STRUCTURAL BIOCHEMISTRY, STEM CELLS AND MOLECULAR BIOLOGY

August 5-6, 2019 | Singapore

Dmitry Malenkov, A S Botin, A Yu Arinbasarova, A G Medentsev, T S Popova, I P Smirnova

Scientific Center of Integrative Medicine, Russia
Sklifosovsky Institute of Emergency Medicine, Russia
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russia
Sklifosovsky Institute of Emergency Medicine, Russia
RUDN University, Russia

Posters & Accepted Abstracts: J Can Res Metastasis

Abstract :

It was discovered that expanded graphite – Nanocomposite of PolyGraphene (PG) obtained after hydro-termic treatment of modified graphite became to be able to interact as sorbent PG with enzyme L-lysine alpha-oxidase from Trichoderma cf. aureoviride Rifai.

It was studied the sorption properties of carbon material as an example of PolyGraphene (PG) concerning enzyme L-lysine alphaoxidase from Trichoderma cf. aureoviride Rifai.

PG - version of ultrafine carbon sorbent, which was developed on the basis of the modified oxygen-containing expanded graphite (OCEG).

L-Lysine α-oxidase (LysOx) is one of the enzymes which are prospective in biotech-nology and medicine due to its antitumor and kinetic properties. An in vivo therapeutic effect was demonstrated on animals with tumor grafts: breast carcinoma SKBR3, Bro melanoma, intestinal cancer HCT116 and LS174T, ovary adenocarcinoma SCOV3, liver carcinoma.

This work aims to immobilize the extracellular L-lysine α-oxidase (LysOx) from Trichoderma cf. aureoviride Rifai VKM F-4268D on PolyGraphene and characterize some properties of adsorbed enzyme. Two types of PolyGraphene were used.

Maximum adsorption equal to 5 or 11 μg protein/ mg of carrier was achieved, a high specific activity comparable to that of a free enzyme to take place.

LysOx adsorbed on PolyGraphene was shown to be a very stable system, namely high stability was revealed in the presence of chaotropic agent (urea) or proteolytic enzymes (pronase, chymotrypsin, trypsin). Thus, the possibility of immobilization of LysOx on graphene with the full conservation of specific activity was shown.

In addition, LysOx is one of the enzymes that is promising in the enzyme therapy of tumors, based on the high sensitivity of tumor cells to the deficiency of growth factors, including amino acids.

Conclusions. Researchers have the theoretical and practical importance, the received results can be applied as matrix both in cancer screening, diagnosis and treatment and in cleaning of already known enzymes.

Biography :

Dmitry Malenkov is now studying Public health management and Economics in Higher School of Economics. Surgeon, cardiothoracic surgeon, oncologist, scientist, public health administrator, co-founder of Scientific center for integrative medicine. Dmitry Malenkov is the author of more than 40 papers, in the field of clinical and experimental medicine.

E-mail: [email protected]

 
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Citations : 681

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