UPLC-MS based integrated plasma proteomic and metabolomic profiling of TSCRAML and its relationship with everolimus treatment

6^th International Conference on UROLOGY AND RENAL HEALTH

May 04-05, 2023 | Zurich, Switzerland

Zhan WANG

Peking Union Medical College Hospital, China

Keynote: Clin Nephrol Res

Abstract :

Aim: To profile the plasma proteomics and metabolomics of patients with renal cysts, sporadic angiomyolipoma (S-AML) and tuberous sclerosis complex related angiomyolipoma (TSC-RAML) before and after everolimus treatment, and to find potential diagnostic and prognostic biomarkers as well as reveal the underlying mechanism of TSC tumorigenesis. Materials and Methods: We retrospectively measured the plasma proteins and metabolites from November 2016 to November 2017 in a cohort of pre-treatment and post-treatment TSC- RAML patients and compared them with renal cyst and S-AML patients by ultra-performance liquid chromatography-mass spectrometer(UPLC-MS). The tumor reduction rates of TSCRAML were assessed and correlated with the plasma protein and metabolite levels. In addition, functional analysis based on differentially expressed molecules was performed to reveal the underlying mechanisms. Results: Eighty-five patients with one hundred and ten plasma samples were enrolled in our study. Multiple proteins and metabolites, such as pre-melanosome protein (PMEL) and S-adenosylmethionine (SAM), demonstrated both diagnostic and prognostic effects. Functional analysis revealed many dysregulated pathways, including angiogenesis synthesis, smooth muscle proliferation and migration, amino acid metabolism and glycerophospholipid metabolism. Conclusions: The plasma proteomics and metabolomics pattern of TSC-RAML was clearly different from that of other renal tumors, and the differentially expressed plasma molecules could be used as prognostic and diagnostic biomarkers. The dysregulated pathways, such as angiogenesis and amino acid metabolism, may shed new light on the treatment of TSC-RAML.
Recent Publications :
1. Wang, Z. (2023) UPLC-MS based integrated plasma proteomic and metabolomic profiling of TSC-RAML and its relationship with everolimus treatment. Front Mol Biosci 2023, 10.
2. Wang, Z. (2022) A multi‑omics study of diagnostic markers and the unique inflammatory tumor micro‑environment involved in tuberous sclerosis complex‑related renal angiomyolipoma. International journal of oncology 2022, 61(5).
3. Wang X. (2022) Analysis of Clinical Features and Next- Generation Sequencing of 12 Tuberous Sclerosis Families in China. Frontiers in medicine 2022, 9:840709.

Biography :

Zhan WANG is a Ph.D. candidate in Urology at Tsinghua University & Peking Union Medical College. He graduated from the Tianjin Medical University in China with a Bachelor of Clinical Medicine and he completed his master’s in Surgery at Tsinghua University & Peking Union Medical College. He was Visiting Ph.D. candidate in Cancer and Immunology at Gustave Roussy (France, Paris), U1015 lab.