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International Journal of Anatomical Variations

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Solomon Demissie1*, Prasad Mergu1, Teshale Fikadu2, Tadios Hailu3, Getachew Abebe1 and Mengistu Warsa4
 
1 Department of Clinical Anatomy, College of Medicine and Health Science, Arba Minch University, Arba Minch, Ethiopia, Email: [email protected]
2 Master of Public Health in Epidemiology, College of Medicine and Health Science, Arba Minch University, Arba Minch, Ethiopia
3 Specialist in Internal Medicine, College of Medicine and Health Science, Arba Minch University, Arba Minch, Ethiopia
4 Medical Doctor, Arba Minch General Hospital, P. O. Box 104, Arba Minch, Ethiopia
 
*Correspondence: Dr. Solomon Demissie, Department of Clinical Anatomy, College of Medicine and Health Science, Arba Minch University, Arba Minch, Ethiopia, Tel: +251921084898, Email: [email protected]

Received Date: Jan 17, 2021 / Accepted Date: Mar 03, 2021 / Published Date: Mar 10, 2021

Citation: Demissie S, Mergu P, Fikadu T, et al. Morphometric assessment of spleen dimensions and its correlates among individual living in Arba Minch town, southern Ethiopia. Int J Anat Var. 2021;14(3):59-65.

This open-access article is distributed under the terms of the Creative Commons Attribution Non-Commercial License (CC BY-NC) (http://creativecommons.org/licenses/by-nc/4.0/), which permits reuse, distribution and reproduction of the article, provided that the original work is properly cited and the reuse is restricted to noncommercial purposes. For commercial reuse, contact [email protected]

Abstract

Introduction: The spleen is a vital lymphoid soft organ that demands constant attention from the clinical point of view. It is multi-dimensional organs that enlarge in all dimensions in disease conditions. The detection of the spleen by palpation on physical examination is not an approval of an enlarged spleen because unenlarged spleen may palpable. Therefore, this study aimed to assess the morphometry of spleen dimensions and its correlates among individuals living in Arba Minch Town by sonographic examinations.

Methods and Materials: Community-based cross-sectional study was conducted in Arba Minch town from February 1 to March 30, 2020. A multistage systematic and simple random sampling technique was applied to select study participants. Data was entered into Epi-Data version 3.1software and exported to SPSS version 20 software for analysis.

Result: A total of 708 individuals, 390 (55.1%) males and 318 (44.9%) females were included in the current study. The mean splenic length, width, thickness and volume were 10.24 cm, 4.79 cm, 3.93 cm, and 109.34 cm³, respectively. The mean spleen length, width, thickness and volumes among males were 10.64 cm, 4.92 cm, 4.05 cm and 119.81 cm³ and among females were 9.75 cm, 4.63 cm, 3.78 cm and 96.50 cm³ respectively. Significant negative correlations were found between age and spleen dimensions. Significant positive correlations were observed between spleen dimensions and height, weight, Body Mass Index, and Body Surface area. But, the correlation between width and body mass index was non-significant (p-value >0.05).

Conclusion and Recommendations: The spleen dimensions were higher in males than females and have a significant positive correlation with height, weight, Body Mass Index and Body Surface Area except for the width. Further studies in different parts of the country have to be done to have normative data on the dimension of Spleen in Ethiopia.

Keywords

Morphometry, Spleen dimensions, Arba Minch

Introduction

The spleen is the largest lymphoid soft organ that lies in the left hypochondrium between the fundus of the stomach and the diaphragm where it is entirely covered by the inferior thoracic rib cage [1]. It extends from the 9th - 11th ribs on the left side with its long axis runs parallel to the 10th rib [2]. Its shape is ovoid with a convex outer diaphragmatic surface and an indented inner visceral surface related to the stomach, left kidney, pancreatic tail, left suprarenal gland and left colic flexure [3,4]. The apex lies in line with the spine of the 10th thoracic vertebra about 4 cm from the midline and the base does not descend beyond the midaxillary line [5].

The spleen is an encapsulated intraperitoneal organ entirely covered with peritoneum except for its hilum where the splenic branches of the splenic artery and vein enter and leave [5,6]. It is supported by a phrenico-colic ligament that originates from the colon from the bottom and anchored to the stomach by gastro-splenic ligament and to the left kidney by a lien renal ligament [6,7].

In diseases condition spleen enlarges at different rates in its dimensions and most easily vulnerable organs [8-10]. A variety of diseases condition alters spleen dimensions, where splenomegaly and its consequence becomes a primary clinical concern in any part of the world [11,12]. Splenomegaly is commonly seen in about 63% of patients with PAH (13), IM [14,15], malaria [16,17], lymphoma [18], kala-azar [19], typhoid fever [20], and others. The altered splenic dimensions and structure during these diseases result in asymptomatic enlargement and complications such as hematoma formation, rupture, hypersplenism, ectopic spleen, and torsion that affect other adjacent organs [21,22]. The splenic atrophy is also another common problem seen in diseases like sickle cell anemia [23] and celiac disease [24]. The progressive atrophy as a result of repeated attacks of vaso-occlusion and infarction caused by these diseases leads to auto splenectomy [23,25,26].

The average dimensions of the spleen are 12.5 cm, 7.5 cm and 2.5 cm in length, width and thickness respectively and 150-200 grams in weight, but its dimensions vary considerably [2,27]. The literature revealed that spleen dimensions are affected by geographical differences, races, nutritional status and anthropometric measurements [28-30]. Due to these variations, a normal range of spleen dimensions varies from country to country where some accept less than 12 cm spleen length [31] and others accept less than 13 cm spleen length in their clinical practice [32], etc. The study conducted in Northern Ethiopia revealed also these variations where normal spleen length ranges vary from 7 cm-13.6 cm [33].

In clinical practice, palpation is commonly used to detect spleen dimensions. However, detection of the spleen by palpation is not reliable because the normal spleen is palpable and a non-palpable spleen is not normal sized at all [34-37]. Similarly, diagnoses of diseases associated with the spleen by physical examination and laboratory findings do not determine parasite load, the extent of diseases and complications caused in case of severe cases [38].

Although, Literatures indicates radiological imaging modalities like ultrasound can detect the extent, complications, and classify the severity of cases, yet enough attention is not given in clinical practice [39]. On sonographic examinations, the spleen is crescent-shaped with the outer margin is smooth and convex and the inner margin is irregularly indented. Its echotexture is homogeneous and more echogenic than healthy liver tissue and markedly hyperechoic compared to kidney tissue [13].

Even though there are several studies in which the spleen dimensions have been analyzed in many countries, there is still a lack of adequate information in African populations including Ethiopia. Therefore, the study is aimed to assess the morphometry of spleen dimensions and to determine its variations with sex, age, height, weight, BMI, and BSA among individuals living in Arba Minch town.

Methods

The community-based cross-sectional study was conducted from February 1 to March 30, 2020, in Arba Minch town, SNNP region which is located at an altitude of 1285 m above sea level and about 437 km far from Addis Ababa.708 individuals (390 males and 318 females) fulfilling inclusion criteria were included in the current study of spleen dimensions. The data collection checklist was developed after reviewing different literature [33,40,41]. The checklist was developed in English and the face validity was assessed by a public health specialist and Anatomist. The checklist was composed of socio-demographic factors (age and sex); anthropometric measurements (height, weight, BSA and BMI) and spleen dimensions (length, width, thickness and volume). A multi-stage sampling technique was used to select study participants. Individuals selected at household levels were sent to the nearest health center or selected private clinics for sonographic examination. History and physical examination of individuals were taken before sonographic examination by clinicians. Then the individuals were sent for sonographic examinations which were performed by two radiology technologists using portable and stationary ultrasound machines equipped with 3.5 MHz curvilinear probes. The subjects were examined in the supine or right oblique positions for overall abdominal examinations to exclude cases associated with the spleen. Then spleen dimensions measurements were performed for apparently healthy individuals. The spleen was scanned during suspended respiration. The splenic length was measured in a longitudinal plane between the dome of the spleen and the splenic tip. The splenic width was also measured in a longitudinal plane perpendicular to the length between the medial and lateral borders of the spleen. The splenic thickness was measured on the transverse plane from the posterior margin to the anterior margin. The volume was calculated using the ellipsoid formula during the analysis [41-52]. The spleen dimensions were measured three times and recorded on the checklist then the average value was taken during analysis [42-45].

Finally, the baselines data including age and sex were recorded for all participants. The height and weight were measured with the stadiometer and weighing machine for all participants respectively. BSA and BMI were calculated during the analysis (Figure 1).

international-journal-anatomical-variations-thickness

Figure 1) The black arrow (D3) shows splenic thickness in a transverse scan.

Inclusion and Exclusion Criteria

Inclusion criteria

All apparently healthy individuals and age greater than 13 years [46,47] Exclusion criteria

• Individuals with a recurrent clinical history of malaria

• Recurrent history of typhoid fever

• Individuals with a history of infections (infectious mononucleosis, kalaazar, endocarditis, sarcoidosis, toxoplasmosis).

• Recent history of upper abdominal surgery

• Individuals with any case finding on sonographic examinations (cirrhosis, melanoma, lymphoma, metastasis, any cystic or solid massive lesions)

• Individuals with Diabetic Mellitus (DM) cases.

• Hypertensive individuals

• Individuals with a history of heart disease

• Individuals with a recent abdominal traumatic condition (within the previous 6 months)

• Pregnant women

Study variables

Dependent variables

Spleen dimensions (length, width, thickness and volume)

Independent variables

• Age

• Sex

• Height

• Weight

• BSA and

• BMI

Operational definitions

Apparently healthy individuals: - defined as the absence of disease based on clinical signs and symptoms of individuals normally assessed by history, physical evaluation and sonographic examinations [48,49].

Data Analysis and Processing

Data was entered into Epi-Data version 3.1software and exported to SPSS version 20 software for analysis. A descriptive summary was used to present the result. Independent- Samples t-test was conducted to compare the mean spleen dimensions among sex and one-way ANOVA was conducted to compare the mean spleen dimensions among age groups. T and F statistics were calculated and P<0.05 was taken as statistically significant. To determine the relationships between the studied parameters, Pearson correlation coefficients were calculated

Results

Socio-demography

A total of 708 (55.1% male and 44.9% female) participants were included in the study with a 100% response rate. The mean age of the study participant was 32.28 ± 13.17 years with a majority of 278 (39.3 %) were between the age group of 21-30 years (Table 1).

Age category in years Sex Total
  Male Female
11-20 72 58 130
21-30 154 124 278
31-40 88 66 154
41-50 28 38 66
Above 50 48 32 80
Total 390 318 708
% 55.1% 44.9% 100.0%

Table 1: Age and sex distribution of the study participants living in Arba Minch town, 2020.

Anthropometric measurements

The mean height, weight, BMI, and BSA of the study participants were 167.56 ± 6.69 cm, 61.75 ± 8.23 kg, 21.98 ± 2.53, and 1.67 ± 0.13 respectively (Table 2).

  N Minimum Maximum Mean Std. Deviation
Height (cm) 708 150.00 188.00 167.5607 6.68908
Weight (kg) 708 44.00 89.00 61.7446 8.22522
BMI 708 14.81 30.67 21.9785 2.53226
BSA 708 1.38 2.10 1.6924 .13246
Total 708        

Table 2: Anthropometric measurement of the study participants living in Arba Minch town, 2020.

Overall spleen dimensions

The mean splenic length, width, thickness, and volume were 10.24 ± 1.458 cm, 4.79 ± 0.99 cm, 3.93 ± 1.05 cm, and 109.34 ± 61.68 cm³, respectively. The dimensions lie with the range of (7.07-13.91) cm in length, (2.32-7.20) cm in width, (2.10-7.09) cm in thickness, and (20.83-349.03) cm³ in volume (Table 3).

  Minimum Maximum     Std. Deviation 95% Confidence Interval  
Mean Std. Error Statistic  Lower bound.  Upper bound.
Length 7.07 13.91 10.2421 .05441 1.44771 10.14  10.35
Width 2.32 7.20 4.7889 .03751 .99811  4.72  4.86
Thickness 2.10 7.09 3.9311 .03960 1.05367  3.85  4.00
Volume 20.83 349.03 109.3381 2.31811 61.68084  104.79  113.89

Table 3:Average spleen dimensions of study participants living in Arba Minch town, 2020.

Comparison of spleen dimensions by sex

The mean spleen length, width, thickness and volume among males were 10.64 ± 1.38 cm, 4.92 ± 1.03 cm, and 4.05 ± 1.09 cm and among females were 119.81 ± 65.32 cm3 and 9.75 ± 1.38 cm, 4.63 ± 0.93 cm, 3.78 ± 0.98cm and 96.50 ± 54.31 cm3 respectively. A significant difference in splenic dimensions was observed in which the dimensions were higher among males than females (Table 4).

  Sex N Mean Std. Deviation 95% CI for Mean T-test P-value
Lower Bound Upper Bound
Length Male 390 10.6433 1.37963 10.5098 10.7859 8.575 0.000
Female 318 9.7500 1.37759 9.6067 9.9044
Width Male 390 4.9155 1.03225 4.8118 5.0181 3.812 0.000
Female 318 4.6336 .93295 4.5366 4.7385
Thickness Male 390 4.0537 1.09359 3.9444 4.1768 3.49 0.001
Female 318 3.7809 .98357 3.6774 3.8898
Volume Male 390 119.8054 65.31931 113.4938 126.7284  5.183 0.000
Female 318 96.5009 54.30818 90.9121 102.8142

Table 4: Independent sample t-test analysis in comparison of spleen dimensions by sex among individuals living in Arba Minch town, 2020.

Comparisons of spleen dimensions with different age groups

Significant variations were observed in all spleen dimensions among age categories of study participants. The lowest value was recorded in age groups of above 50 years (9.36 cm, 4.35 cm, 3.56 cm and 86.33 cmᶾ for length, width, thickness, and volume respectively) and the highest in age groups of 31-40 years (10.76 cm, 4.91 cm, 4.2 cm and 126.02 cmᶾ for length, width, thickness, and volume respectively). As the age increases all spleen dimensions were found to decrease above the age of 40 years (Table 5).

  Age (in a year) N Mean SD 95% CI F P-value
Lower B. Upper B.
Length 11-20 130 10.14 1.21 9.93 10.35 16.53 0.000
21-30 278 10.37 1.31 10.23 10.53
31-40 154 10.76 1.49 10.52 10.99
41-50 66 9.72 1.57 9.34 10.11
>50 80 9.36 1.56 9.02 9.71
Total 708 10.24 1.45 10.14 10.35
Width 11-20 130 4.90 0.90 4.75 5.06 6.43 0.000
21-30 278 4.85 0.92 4.75 4.96
31-40 154 4.91 1.08 4.74 5.08
41-50 66 4.54 1.04 4.28 4.80
>50 80 4.35 1.08 4.11 4.59
Total 708 4.79 0.99 4.72 4.86
Thickness 11-20 130 3.99 0.87 3.85 4.15 5.94 0.000
21-30 278 3.91 0.99 3.79 4.02
31-40 154 4.20 1.21 4.01 4.39
41-50 66 3.73 1.08 3.47 3.99
>50 80 3.56 1.05 3.32 3.79
Total 708 3.93 1.05 3.85 4.01
Volume 11-20 130 109.59 51.77 100.61 118.58 6.55 0.000
21-30 278 109.70 54.92 103.22 116.19
31-40 154 126.02 71.26 114.68 137.37
41-50 66 96.26 69.01 79.29 113.22
>50 80 86.33 64.20 72.04 100.61
Total 708 109.34 61.68 104.79 113.89

Table 5: One-way ANOVA analysis of different age groups in comparison of spleen dimensions among the study participants living in Arba Minch town, 2020.

Correlation analysis of spleen dimensions

Pearson’s correlation analysis showed that a significant correlation was observed among all evaluated spleen dimensions and height, weight, BSA, and BMI except the width which showed non-significant correlations with BMI (p>0.05). The highest positive correlation was observed between spleen length and height of participants (r=0.518) while the lowest was between spleen thickness and BMI of participants (r=0.111). Spleen length, width, thickness and volume were negatively correlate with age (r=-0.183, -0.165, -0.107 and -0.100 respectively) (Table 6).

  Height Weight BMI BSA Length Width Thickness Volume Age
Height R 1 .516** -.112** .693** .518** .411** .354** .437** .002
Sig. (2 -  tailed)   .000 .003 .000 .000 .000 .000 .000 .960
Weight R .516** 1 .790** .975** .442** .307** .312** .374** .309**
Sig. (2-tailed) .000   .000 0.000 .000 .000 .000 .000 .000
BMI R -.112** .790** 1 .636** .143** .066 .111** .120** .363**
Sig.(2-tailed) .003 .000   .000 .000 .080 .003 .001 .000
BSA R .693** .975** .636** 1 .506** .364** .354** .427** .262**
Sig. (2-tailed) .000 0.000 .000   .000 .000 .000 .000 .000
Length R .518** .442** .143** .506** 1 .707** .621** .803** -.183**
Sig. (2-tailed) .000 .000 .000 .000   .000 .000 .000 .000
Width R .411** .307** .066 .364** .707** 1 .690** .864** -.165**
Sig. (2-tailed) .000 .000 .080 .000 .000   .000 .000 .000
 Thickness R .354** .312** .111** .354** .621** .690** 1 .900** -.107**
Sig. (2-tailed) .000 .000 .003 .000 .000 .000   .000 .004
Volume R .437** .374** .120** .427** .803** .864** .900** 1 -.100**
Sig. (2-tailed) .000 .000 .001 .000 .000 .000 .000   .008
Age R .002 .309** .363** .262** -.183** -.165** -.107** -.100** 1
Sig. (2-tailed) .960 .00 0 .000 .000 .000 .000 .004 .008  

Table 6: Pearson’s correlation analysis of spleen dimensions with age, height, weight, BMI and BSA among individuals living in Arba Minch town, 2020.

Discussion

This study describes the morphometry of spleen dimensions; compare the presence of a significant difference between sex and age as well as dimensional correlations with anthropometric measurements. The mean of spleen dimensions was also compared with other studies and consistency and the difference was noted in the current study. The sonography assessment of spleen dimensions provides essential inputs for clinicians in daily clinical practice for proper diagnosis of splenomegaly [32,44,50-55]. The result of this study gives reference values which helps radiologist for appropriate diagnosis of diseases associated to splenomegaly and splenic atrophy, for the surgeons to appropriately design surgical cases associated to spleen, for hematologist and immunologist for proper diagnosis of various gastrointestinal and hematological diseases and forensic studies [56-59].

The mean of spleen length at 95% CI with SD was 10.24 ± 1.45 cm which is consistent with studies conducted in Russia and Kashmir [60,61]. But, less than from study conducted in Turkey [42], Bangladesh, Jordan and North India [8,41,62,63] and greater than from study conducted in Nepal, Nigeria, Sudan and Northern Ethiopia [29,33,42,64].

The mean of spleen width was 4.79 ± 0.998 cm which is less than from study conducted in Bangladesh, Nepal, Kashmir, North India and Nigeria [8,42,44,61,63,64] and greater than from study conducted in Sudan and Northern Ethiopia [29,33].

The mean of spleen thickness in the current study was 3.93 ± 1.054 cm which is consistent with the study conducted in Ethiopia [33]. But less than from studies conducted in Russia, Bangladesh, Jordan, Nepal, north India, and Nigeria [8,41,42,44,60,63,64] and is greater than from a study conducted in Sudan [29].

The mean spleen volume was 109.34 ± 61.68 cm³ which is less than from studies conducted in Saud Arabia, Russia, Jordan and Nepal [41,53,60,64,65] but greater than from the study conducted in Sudan and Ethiopia [33,40].

The overall dimensional difference of the current study from studies conducted in other areas may be due to age group difference, sample size difference, anthropometric measurements, geographical differences, nutritional status, physical exercise, and race difference which were stated in different literature [32,44,59,66-69].

The fact that fewer average total red cell mass among females and due to genetic factor splenic dimensions was lower in females [31,70]. This is supported by our findings where all dimensions were significantly larger in males than females. This is consistent with the study conducted in Turkey, Saudi, Nigeria and Sudan [40,53,53,71,72]. But, a non-significant difference in spleen volume was observed in a study conducted in Nepal [64] which is due to the small sample size and age range difference where 1-90 years were included in the study of Nepal. Also, the length was higher among females than males in the study conducted in Egypt [73] which is maybe due to nutritional status where Egyptian culture accepts excess weight gain among women than men due to fertility considerations [74,75].

The spleen dimensions decrease in older age due to a decrease in the number and size of B cell follicles of the white pulp of the spleen which decreases with a decrease of germinal center in older age groups [76-78]. This is supported by our finding where the lengths, width, thickness, and volumes were significantly increased up to the age of 40 years then decrease in older ages. This is consistent with the study conducted in Iraq, western Nepal, and India [32,45,54,55,79,80]. But, this different from the study conducted in Pakistan, Jordan, and Nigeria [41,42,68,72]. The difference is maybe due to nutritional status where larger anthropometric measurements and obesity were observed in the study of Pakistan [68], Jordan [41] and Nigeria [42,72].

From a physiological perspective as individuals’ body parameters (height, weight, BMI, and BSA) increase, the blood volume increase and requiring larger spleens for filtration [41]. This is supported by our finding where all dimensions were positively correlated with height, weight, BMI, and BSA (p <0.05). This is consistent with the study conducted in Jordan, USA, India, Sudan [29,31,37,41,61,63,79]. But, different from the study conducted in Turkey, Nigeria and Egypt [42,62,68,73]. This may be due to nutritional status where the study participants in the study of Turkey, Nigeria and Egypt were overweight than ours. The non-significant correlations observed between spleen width and BMI in the current study (P=0.080) is consistent with the study conducted in Sudan [40].

Limitation of the Study

• The current study does not include the history of the physical exercise of the participants.

• A laboratory finding of study participants is not known.

Conclusion

The mean value of spleen dimensions of the Arba Minch town community was 10.24 cm, 4.79 cm, 3.93 cm, and 109.34 cm³, in length, width, thickness, and volume respectively. The study indicated that there is a significant morphometric difference in splenic dimensions between males and females. Only the width shows a non-significant correlation with BMI and significant negative correlations was seen between age and spleen dimensions. This study gives baseline information for clinicians as well as for academicians about the morphometric variation of spleen dimensions which helps in diagnosing pathological cases associated with spleen both splenomegaly as well as splenic atrophy.

Abbreviations and Acronym

AP: Antero-Posterior; BMI: Body Mass Index; BSA: Body-Surface Area; CI: Confidence Interval; Cm: Centimeter; CSA: Central statistical agency; CT Scans: Computed Tomography Scans; IM: Infectious Mononucleosis; Kg: kilogram; M: Meter; MH: Megahertz., MRI: Magnetic resonance imaging; P: P-value; PAH: Pulmonary arterial hypertension; R: Pearson correlation coefficient; RT: Radiologic Technologists; SD: Standard Deviation; SNNP: Southern Nations, Nationalities and peoples; SPSS: Statistical Package for the Social Sciences; US: Ultrasonography; USA: United States of America

Declarations

Ethical approval and consent to participate

We obtained ethical approval from Institutional Review Board (IRB), College of Medicine and Health Sciences, Arba Minch University, with reference number of IRB/165/2012. Written informed consent was obtained from each study participant. We informed all the participants about the purpose of the study and the importance of their participation in the study. Privacy and confidentiality of information were properly kept.

Consent for Publication

Not applicable

Competing Interests

The authors declare that they have no competing of interest.

Funding

No specific funding for the study

Availability of Data and Materials

All relevant data are included in the article. The dataset of this study are available from the corresponding authors upon reasonable request.

Authors’ Contributions

SD conceptualize and designed the study, conducted the analysis, interpret and write the first draft of the paper for publication. PM, TH, TF, GA and MW were involved in the data analysis and interpret the data, and reviewed the manuscript for publication. TF and TH supervise the data collection process. All authors read and approve the final manuscript before submission.

Acknowledgement

The authors would like to acknowledge the Arba Minch University for allowing the study to be conducted, and the data collectors for their willingness to collect the data. We would like also to thank Arba Minch town health office, Arba Minch town health centers, private clinics for their full cooperation to take my study in their institutions, facilitating study setting and for the unreserved offer of their medical equipment required for the data collection. Lastly, we would like to thank our study participants for their full cooperation to participate in our study.

References

 
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