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OBJECTIVE: To determine the optimal radiation dose and observe the influence of radiation sterilization on spirulina polypeptide powder. METHODS: The polypeptide powder of spirulina was sterilized using a 60Coγ ray. RESULTS: Radiation sterilization had significant effects on spirulina polypeptide powder, which was more effective with increasing radiation dose. Taking the sterilization rate and peptide content into account, the most effective radiation dose for sterilization was 9 kGy. CONCLUSION: Reasonable conditions for sterilization have the effect of sterilization on spirulina polypeptide powder.