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Rapid detection of yeast infections aids in the administration of timely antifungal treatment. The utility of chromogenic medium, slide culture technique, and Vitek2 Compact (V2C) was investigated in this work. The study includes a total of 173 clinical isolates of yeast species. An algorithm was created and followed to identify such isolates in a regular clinical microbiology laboratory. Candida albicans, Candida tropicalis, Candida krusei, Candida parapsilosis, and Trichosporon asahii were all identified using chromogenic media. The use of chromogenic medium helped to identify "multi-species" yeast infections. C. pelliculosa, C. utilis, C. rugosa, C. glabrata, and C. hemulonii were all unable to be identified using the medium. All pseudohypae non-producing yeast species were distinguished using Vitek 2 compact (V2C). The methodology used proved effective in identifying and diagnosing yeast infections, including multi-species yeast infections, in a timely manner.