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Comparative anatomy is a foundational discipline in biological sciences that examines the similarities and differences in the anatomical structures of organisms. This field provides critical insights into evolutionary relationships, developmental biology, and functional morphology. Through a comparative lens, anatomists can trace homologous structures across species, assess adaptations, and reconstruct evolutionary lineages. This paper explores the historical background, major methodologies, and contemporary applications of comparative anatomy, while also addressing its intersection with modern technologies like genomics and imaging techniques.