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This research paper is an investigation into the effects of fructose and glycerol as plasticizers in cassava bioplastic production. The experiments were carried out at the University of Eastern Africa, Baraton Department of Chemistry. The objectives of the research were to produce cassava-based bioplastics in the University of Eastern Africa, Baraton Chemistry Department Laboratory, to investigate the use of fructose and glycerol as plasticizers in the production of the cassava-based bioplastics and to conduct physical and chemical quality tests on the bioplastics to determine which plasticizer is best for industrial use. A Randomized Complete Block Design (RCBD) was used in the experiments. The parameters measured were film thickness, density, moisture content, solubility in water, water absorption, swelling index, and biodegradability test. Overall, fructose as a plasticizer is recommended over glycerol and over fructose and glycerol.