44 2033180199
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
Journal of Experimental and Clinical Microbiology

Sign up for email alert when new content gets added: Sign up

Molecular typing of Mycobacterium tuberculosis isolates from Qinghai province of northwest china by spoligotyping and 15-locus MIRUVNTR

Author(s): Bin Li, ZhaofenWang*, Yongcheng Ma, Haican Liu, Xiuli Shen, Binzhong Ma, Mingxia Jiang, Yuanyuan Zhang, Xiaodong Su, Wenqi Du, Haijing Wang, Xiuqin Zhao and Kanglin Wan

Tuberculosis (TB) is a major public health concern in Qinghai which located in the northeastern region of Qinghai-Tibet Plateau and characterized by low oxygen and low pressure. This study was conducted to explore the genotypes of Mycobacterium tuberculosis (MTB) in this high altitude region. A total of 251 MTB strains were collected and genotyped by spoligotyping and 15- loci mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) typing. Spoligotyping of 251 MTB isolates revealed 49 distinct spoligotype patterns and Beijing lineage was found to be the most dominant MTB strain including 74.0% (185/251) of total isolates. 217 isolates grouped into 15 clusters with the clustering rate of 86.5% (217/251). While 15-loci MIRU-VNTR distributed 251 isolates into 4 groups containing 238 different MIRU-VNTR profiles, Group II was the largest group representing 91.9ï¼ÂÂ… (230/251) of isolates. Clustering rate by MIRUVNTR was 85.7% (215/251). Spoligotyping and MIRU-VNTR matched well in lineage classification. We concluded that 15-loci MIRU-VNTR has more abundant diversity than spoligotyping and would be useful to trace transmission routes and sources of infections.

Full-Text | PDF
Google Scholar citation report
Citations : 46

Journal of Experimental and Clinical Microbiology received 46 citations as per Google Scholar report