Prunus persica (L.) leaves and Trichosanthes dioica (R.) seeds are mentioned in various Asian traditional texts as a drug used for anthelmintic purpose ethno pharmacologically. The objective of the present study was investigation of anthelmintic activity of different extracts of P. persica leaves & T. doica seeds along with optimization of dose. Nematodes, Ascaridia galli were used to carry out experiments for anthelmintic activity. Piperazine citrate was used as a standard drug. Time required for paralysis and death (lethal time) of worms were noted for each sample of extracts of both plants as well as standard. The results demonstrated that treatment with both plant extracts significantly (P<0.01) paralyzed and killed A. galli. The activity was found to be increased with dose. Ethanol and ethyl acetate extracts activity at 60 mg/ml concentration were comparable to the well known anthelmintic agent Piperazine citrate (10 mg/ml). In conclusion, the use of the leaves of P. persica & seeds of T. dioica as a anthelmintic has been confirmed. Further studies were carried out by optimizing different ratio of ethanol extract of both plants in combination to get more prominent efficacy as a herbal anthelmintic by using minimum concentration of extracts of plants