Optimization of the extraction yield of plant waxes by the development of experimental designs

Fruits peel of mangifera indica and the leaves of the borassus aethiopum are new sources of waxes not exploited industrially. The main extraction yield parameters were optimized using response surface methodology. It is so important to maximize the extraction yield of mangifera indica and borassus aethiopum in order to better exploited those resources. In fact, a screening design have been realized according to PlackettBurman method. The results of tests conducted in optimal conditions, showed a significant increase in extraction yield for both models. The analysis of products by thin layer chromatography showed that the main compounds of waxy extracts are alkanes, alkenes, squalene and sterolester. The presence of others tasks such as free fatty acids, diglycerides, phospholipids, monoglycerides, sterols and others compounds of unknown nature show as well that the plant waxes of this study are of lipids of complex composition like the majority of plant waxes met in the literature