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Anatomical variations are a fundamental aspect of human biology, often overlooked in traditional anatomical education. These deviations from the “standard” anatomical structure, while typically benign, can pose significant challenges in surgical practice. This paper provides a comprehensive review of the most clinically significant anatomical variations encountered across major surgical disciplines. It emphasizes the necessity for preoperative recognition, discusses their embryological basis, and evaluates modern imaging techniques and intraoperative strategies that help mitigate surgical risk. Increased awareness and understanding of these variations are crucial for improving surgical outcomes, reducing iatrogenic injuries, and personalizing patient care.