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Shanghai Jiao Tong University, China
Posters & Accepted Abstracts: J Clin Gen Genomics
Traditional cell-based medical therapeutics are usually performed with the enzymes (trypsin) to transfer cells from culture dishes to the wounds, which destroy the extracellular matrix (ECM) and reduce cell efficacy, cell activity and cell integrity. Technologies for transferring cell sheets with a completely integrated ECM and intact cell–cell junctions have been established for applications in surgical operations and regenerative medicine, such as ocular regeneration, cardiac tissue regeneration and bladder augmentation. Developments of cell sheet engineering are in progress aimed at commercial applications. However, there are still a lot of problems in terms of practical operation. For example, the resultant cell sheets are easily crumpled, wrinkled and tangled due to the uncontrollable detachment and disturbance from hydrodynamic forces. For more delicate clinical trials, a unifying cell sheet operation with careful detaching direction could facilitate consistent, ordered and reproducible production. Strategies to harvest cell sheets along a predetermined direction are significant to address this issue but still in the infancy.
A PEDOT film with gradient thickness was designed to provide a gradient photothermal field for locally dissolving a type I collagen layer. Type I collagen is comprised of cross-linked tri-helical peptides and forms a gel at relatively low temperature, which promptly dissolves on heating beyond 42°C. Its dissociation rate is linearly related to temperature. Here, under a gradient photothermal field, precisely directed collagen dissolution was expected to guide detachment of the cell sheet. Intuitively, as this novel scenario provides an opportunity for precisely directing cell sheet detachment, it can be envisaged as greatly facilitating broader commercial application of next-generation cell sheet engineering.
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