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Nelli Hovhannisyan, B G Babayan, A S Sargsyan, S A Bagdasaryan and A M Hovhannisyan
Scientific and Production Center“Armbiotechnology” NAS RA, Armenia National Polytechnic University of Armenia, Armenia Yerevan State University, Armenia
Posters & Accepted Abstracts: J Clin Microbiol Infect Dis
The emergence of multidrug-resistant (MDR) P. aeruginosa is a critical problem in medical practice, however the key features involved in the emergence and spread of MDR P. aeruginosa remain unknown. Plasmids play the main role in rapid dissemination of resistance and virulence genes by mechanism of genes horizontal transfer. The spread of antibiotic resistance (ABR) has close relations with stability of plasmids possessing that features. To control the process of dissemination of MDR strains it is important to understand how resistance genes remain stable in environment.
The stability of plasmid isolated from MDR soil Pseudomonas and Xanthomonas strains has been studied. All studied strains are included in culture collection of MDC SPC “Armbiotechnology NAS RA. The MDR and the presence of blaOXA-10, aac(6’)II, aph(3’)IV in the genome of these strains were studied earlier.
Plasmids isolated from MDR strains were used for transformation of sensitive P. aeruginosa 9056 strain and E. coli DH5α strain. Antibiotic resistant colonies have been selected and passed over series of cultivation in antibiotic-free medium in order to determine loss of plasmids (resistance). Results are presented in the Table.
Plasmids containing in P. aeruginosa 9056 transformants have demonstrated high level of stability. However, E. coli transformants have lost ABR after transfer to antibiotic medium. Thus, probably stability of Pseudomonas and Xanthomonas ABR plasmids is under the host control.
Nelli Hovhannisyan has her expertise in biotechnology. The one of her scientific interests is combat against multidrug resistance bacteria maintenance and spread in environment. She has experience in research, teaching and administration in education institutions including Universality. As a head of Ecological safety lab, she manages the projects connected with ecology and biotechnology.
E-mail: nelliog@yahoo.fr
