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This research involved synthesis and characterization of Silver Nano particle (AgNP) for using as antibacterial agent. AgNP was achieved from Silver Nitrate reduced with Sodium Tripolyphosphate. The synthesized AgNP were characterized with AFM and SEM. The morphological investigations revealed 100- 150nm sized particles homogenously from the yielded suspension. The antibacterial investigation of synthesized AgNPs were performed against the commonly available poultry bacteria, such as- Staphylococcus, E. coli and Salmonella isolated from different bird types from two live bird markets surrounding Bangladesh Agricultural University campus. The antibiogram profiling of synthesized Ag NP along with commercially antibiotic disk through standard disk diffusion method was employed against representatives of Staphylococcus (n=68), coli (n=97) and Salmonella (n=91) isolated from a total of 120 cloacal swab samples collectedfrom Broiler (n=48), Sonali (n=48) and Deshi chi cken (n=24) from K.R. and Kewatkhali live bird market surrounding the BAU campus. The antibiogram assays revealed the zone of inhibition surrounding to Ceftriaxone (26.99±0.2), Ciprofloxacine (21.57±0.15), Gentamycin (23.59±0.2) and resistance to Amoxicillin (0.00), Tetracycline (7.23±0.0 6) while Ag(22.93±0.38) was sensitive against all the isolates. Finally, MIC for the synthesized AgNP was determined for each of those bacteria. AgNP proved sensitivity to bacteria even resistant to Amoxicillin and Tetracycline. This sensitivity of AgNP against poultry bacteria holds promise to be used as a new antibacterial in poultry industry.