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Journal of Nanoscience and Nanomedicine

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Green synthesis, characterization and kinetic analysis of cellular uptake of glycans coated silver nanoparticles

Author(s): Daniela Manno*, Alessandro Buccolieri, Gabriele Giancane, Luciana Dini and Antonio Serra

Silver nanoparticles were obtained via totally green synthesis from silver nitrate using glucose as reducing agent and sucralose as capping agent. Physico-chemical characterization of AgNPs was performed by UV-Visible absorption and transmission electron microscopy (TEM) in order to correlate biological/toxicological responses with AgNPs features. A detailed study of silver nanoparticles (AgNPs) internalization process in human epitheloid cells of cervical cancer (HeLa) was performed versus time. Graphite furnace atomic absorption spectroscopy (GF-AAS) was used in order to assess the silver concentration effectively internalized by the cells. A kinetic model was developed to interpret the results.

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