44 2033180199
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
Journal of Immune Disorders & Therapy

Sign up for email alert when new content gets added: Sign up

Outer Membrane Proteins of Salmonella Typhimurium as an Adjuvant in Rabies Vaccine

Author(s): Iman Ibrahim Negm , Yasser M Ragab , Aly Fahmy Mohamed

Purpose: The objective of the present study was to evaluate the immune-enhancing potential of Salmonella Typhimurium Outer Membrane Proteins and alum as adjuvants towards inactivated Vero cells rabies vaccine (FRV/K2).

Materials and methods: Six groups of female Sprague Dawley albino rats (10 /group) were used in the evaluation of immunogenicity and safety of vaccines and adjuvants. Total IgG, secreted IFN-γ, and the percentage of proliferated CD4+ and CD8+ T-cells were measured. Biochemical analysis and histopathological examination were used to test safety profiles.

Results: OMP adjuvanted rabies vaccine (FRV/K2 +OMP) induced significantly higher neutralizing antibodies on day 21 post-vaccination relative to free (FRV/K2) vaccine and alum adsorbed vaccine (FRV/K2+alum) . (FRV/K2 +OMP) induced a significantly higher level of IFN-γ on day 14 post-vaccination. CD8+ T cells were significantly higher post-vaccination with reference (RV), free (FRV/K2), and (FRV/K2 +OMP) than (FRV/K2+alum). On the contrary, CD4+ T cells were significantly elevated post-vaccination with (FRV/K2+alum) at P < 0.05. Biochemical analysis and histopathological examination revealed that OMP could be used safely as an adjuvant for the development of more effective rabies vaccines.

Conclusion: Outer membrane proteins adjuvanted rabies vaccines would be beneficial to induce rapid neutralizing antibodies and essential cytokines. Keywords: Rabies, Outer membrane proteins, Adjuvant, Vaccine, Immune response.

Google Scholar citation report
Citations : 2

Journal of Immune Disorders & Therapy received 2 citations as per Google Scholar report