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General Surgery: Open Access

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Research on diabetes mellitus: The results and quality of pancreatic islet cells isolated from surgical samples

Author(s): Denial Smith*

For diabetes research, a substantial number of high quality human islets must be isolated. Since human islets are often only available from brain dead donors, study is made challenging by their scarcity. After surgical excision, pancreatic tissue that is discarded may make a suitable alternate supply of islet cells. We separated islets from surgical materials that were discarded in order to verify this idea. We next assessed the quantity, quality, and preparations of islet cells. Utilizing the automated Ricordi technique, 82 segmental pancreases were processed, and islet yield and quality were examined. 32 individuals with diabetes made up the group, with a mean age of 54.6 patients. Following purification, partly resected pancreases produced an average of 71.5 21 percent pure Islet Equivalents (IEQs) and 2546 IEQ/g of digested pancreas. Diabetes (p=0.0046) and the lobe employed (p=0.0156) were found to substantially impact islet yield by multivariate analysis.

Despite the fact that these outcomes depended on the islet quality, islets transplanted into diabetic mice showed good survivability, in vitro glucose responses, DNA/RNA quality, mitochondrial activity, and glucose management. Even after being frozen, isolated cells still exhibited good vitality and functionality. According to our research, pancreatic tissue that is discarded during surgery might be a useful source of islets for studies into diabetes.

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Citations : 11

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