Opinion
The rseC gene's essential function for autotrophy and the significance of a functional electron balance for nitrate reduction in Clostridium ljungdahlii are shown by genetic evidence
Author(s): Katie Harley*
Conversion from gaseous sources like carbon dioxide and hydrogen. In a previous study, autotrophy was lost when RNF-complex genes were disrupted, although glycolysis still allowed for heterotrophy. Additionally, it was demonstrated that nitrate supplementation might remove the energy restriction during autotrophy, leading to increased cellular growth and ATP output. Here, we deleted the putative RNF regulator gene rseC, the putative nitrate reductase gene cluster, and the RNF complex-encoding gene cluster rnfCDGEAB using CRISPRCas12a. The entire loss of autotrophy caused by the deletion of either rnfCDGEAB or rseC may be recovered by plasmid-based complementation of the lost genes. We noticed a transcriptional inhibition of the RNF-gene cluster in the rseC-deletion strain during autotrophy, and we looked into the distribution of the rseC gene across Acetogenic bacteria. We contr.. Read More»
DOI:
10.37532/puljem21.3 (1).1-2
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