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Awatif Al-Judaibi and Sara Al-Alee
King Abdulaziz University, KSA
Posters & Accepted Abstracts: J Sex Reprod Med
This study aimed to compare the antimicrobial activity of Phoenix dactylifera and Ziziphus spina-christi ethanol extracts in terms of their biochemical and molecular effects on Candida albicans. These effects were evaluated regarding intracellular sterols, permeability of the cell membranes, and morphological characteristics determined by scanning electron microscopy (SEM). Energy-dispersive X-ray spectroscopy (EDAX) analyses were also conducted in addition to assessment of the changes in TEF1: QRTTEF1, CaERG1: ERG1, CdERG12: CdERG1, and ERG25: ERG25 genes. The results showed that sterols increased by 1.096% and 0.588% with treatment by P. dactylifera and Z. spina-christi, respectively, compared to the untreated cells. The ethanol extracts were effective on C. albicans permeability by reducing the cell membranes permeability. The SEM and EDAX analyses showed cell cavities and shrinkage of the cell wall. In addition, the quantity of cells was decreased to a few abnormal cells compared to the untreated cells. Yttrium was detected in the cells treated with Z. spina-christi, and high levels of osmium were detected in the cells treated with P. dactylifera. The gene sequence showed gaps and mismatches on ERG1F, ERG1R, ERG12F, ERG12R, and ERG25F genes after treatment with P. dactylifera and Z. spina-christi compared to untreated cells. The results were highly significant (p ΓΆΒ?Β¤ 0.01), and we concluded that ethanol extracts of P. dactylifera and Z. spina-christi have an antimicrobial effect on several targets in yeast cells.