Mineralocorticoids regulate sodium transport by modulating the expression and degradation of epithelial sodium channels (ENaC) through activation of mineralocorticoid receptors (MR). Mineralocorticoid mediated neural mechanisms acting through MR and ENaC are crucial to the development and maintenance of hypertension in SHR and Dahl S rats on high salt diet. Where and how this occurs in the brain is not yet known. The purpose of this study was to delineate MR and ENaC expression in the brain. Immunohistochemistry was done using MR and aENaC specific antibodies. MR staining was noted in the Median preoptic (MnPO), Paraventricular (PVN) and Supraoptic (SON) hypothalamic nuclei which are important in autonomic regulation of BP. Prominent diffuse staining was present in the lining epithelia of the ventricles and choroid plexus (CP) and in circumventricular organs like the Organum vasculosum of lamina terminalis (OVLT) and Sub fornical organ (SFO). aENaC showed widespread distribution throughout the brain including the PVN, SON, ventricular lining and the choroid plexus. Similar distribution of MR and aENaC in the hypothalamic nuclei and CSF secreting epithelium is consistent with the concept that they might be involved in regulating neuronal activity and CSF sodium concentration.